Name: WANG Haoyi

Subject: Development Biology

Tel/Fax: +86-10-82619213

E-mail: wanghaoyi@ioz.ac.cn

Address: The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences,1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China

Resume

Dr. Wang has an interdisciplinary training in genetics, molecular biology, and stem cell biology. As a doctoral and post-doctoral researcher, Dr. Wang has worked on the development of a variety of genome engineering technologies, including transposon-based “Calling Card” method for determining the genome-wide binding locations of transcription factors, TALEN-mediated genome editing in human pluripotent stem cells and mice, CRISPR-mediated multiplexed genome editing in mice, and CRISPR-mediated gene activation in human cells.

In 2014, he established his own lab at the Institute of Zoology, Chinese Academy of Sciences (Beijing, China). Since then, Wang lab has developed Zygote Electroporation of Nuclease (ZEN) method to generate genetically modified mouse models with high throughput and efficiency, Casilio method to regulate gene transcription, as well as method to generate CAR-T cells with multiplex gene editing. Wang laboratory focuses on developing novel technologies to achieve efficient and specific genome engineering, and applying them to study the function of genes and establish novel therapeutic methods.

2014-Present Principle Investigator, Group leader of Genome Engineering Technology.

2009-2014 Postdoctoral Associate with Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology.

Development and application of site-specific nucleases on genome engineering of hES, hiPS cells and mouse.

2003-2009 Doctoral Candidate, with Mark Johnston and Rob Mitra, Washington University in St. Louis.

Development and application of transposon "calling cards", a novel method for determining thegenome-wide binding locations of transcription factors.

2001-2003 Masters Candidate, Laboratory of Dr. Susan Hoffman, Miami University.

Detailed analysis of the organization and evolution of the Cyp2 gene family cluster in mouse.

1997-2010 B.S. Candidate, Biology, Xiamen University, Fujian, China

Research Interests

1. Development of novel tools for genome engineering.

2. Establishment of novel therapeutic methods using gene editing.

3. Study the mechanism of X-chromosome inactivation in human using stem cell and CRISPR technologies.

Selected Publications

1. Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H^#. (2017). CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells. Frontiers of Medicine. 11(4):554-562.

2. Xiang G, Zhang X, An C, Cheng C, Wang H^#. (2017). Temperature effect on CRISPR-Cas9 mediated genome editing. Journal of Genetics and Genomics. 44(4):199-205.

3. Liu X, Zhang Y, Cheng C, Cheng WA, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H^#. (2017). CRISPR-Cas9 mediated multiplex gene editing in CAR-T cells. Cell Research. 27(1):154-157.

4. Burgess S, Cheng L^#, Gu F, Huang J, Huang Z, Lin S^#, Li J, Li W, Qin W, Sun W, Songyang Z, Wei W^#, Wu Q, Wang H^#, Wang X, Xiong JW, Xi J, Yang H, Zhou B, Zhang B. (2016). Questions about NgAgo. Protein Cell. 7(12):913-915.

5. Wang W, Kutny PM, Byers SL, Longstaff CJ, DaCosta MJ, Pang C, Zhang Y, Taft RA, Buaas FW, Wang H^#. (2016). Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increased the efficiency of model creation. Journal of Genetics and Genomics. 43(5):319-327.

6. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H^#. (2016). Generating Mouse Models Using CRISPR-Cas9 Mediated Genome Editing. Current Protocols in Mouse Biology. 6(1):39-66.

7. Cheng AW^*#, Jillette N^*, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H^#. (2016). Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Research. 26(2):254-257.

8. Wiles MV, Qin W, Cheng AW, Wang H^#. (2015). CRISPR-Cas9 mediated genome editing and guide RNA design. Mammalian Genome. 26(9-10):501-510.

9. Qin W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen YG, Wang H^#. Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease. Genetics. 200(2):423-430.

10. Theunissen T^*, Powell B^*, Wang H^*, Mitalipova M, Faddah D, Reddy J, Fan Z, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty M, Young R, Gray N, Jaenisch R. (2014). Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency. Cell Stem Cell. 15(4):471-487.

11. Yang H, Wang H, Jaenisch R. (2014). Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nature protocols. 9(8):1956-1968.

12. Maetzel D^*, Sarkar S^*, Wang H^*, Mosleh LA, Cheng AW, Xu P, Gao Q, Mitalipova M, Jaenisch R. (2014). Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells. Stem Cell Reports. 2(6):866-880.

13. Cheng AW^*, Wang H^*, Yang H, Shi L, Katz Y, Rangarajan S, Theunissen TW, Shivalila CS, Dadon DB, Jaenisch R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, a RNA-guided transcriptional activator system. Cell Research. 23(10):1163-1171.

14. Yang H^*, Wang H^*, Shivalila CS^*, Cheng AW, Shi L, Jaenisch R. (2013). One-step generation of mice carrying reporter and conditional allele by CRISPR/Cas mediated genome editing. Cell. 154(6):1370-1379.

15. Faddah D, Wang H, Buganim Y, Cheng AW, Jaenisch R. (2013). Expression of Nanog is biallelic and equally variable as other pluripotency factors. Cell Stem Cell. 13(1):23-29.

16. Wang H^*, Hu YC^*, Markoulaki S, Welstead GG, Shivalila CS, Cheng AW, Pyntikova T, Dadon D, Voytas DF, Bogdanove AJ, Page DC, Jaenisch R. (2013). TALEN-mediated editing of the Mouse Y Chromosome. Nature Biotechnology. 31(6):530-532.

17. Wang H^*, Yang H^*, Shivalila CS^*, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. (2013). One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas mediated genome engineering. Cell. 153(4):910-918.