Nankai University, China

State key Laboratory of Chemical Medicinal Biology

Dr. Ling Shuai

38 Tong Yan Road, Jinnan district, Tian Jin, China, 300350

Laboratory building D420

Email: lshuai@nankai.edu.cn

Biography:

  Dr. Ling Shuai got a tenure track as a principal investigator at State key Laboratory of Chemical Medicinal Biology in Nankai University, China since 2015. He received his Ph.D from Northeast Agricultural University, China in 2013 and completed post-doctoral training at Institute of zoology, Chinese Academy of Sciences in 2015. He worked on developing and application of mouse androgenetic haploid embryonic stem cells (ESCs) for genetics research and transgenic animal producing. Now, Ling mainly focuses on developing new haploid cells (haploid monkey and mouse NSCs, haploid human and mouse TSCs) and combining with genetic screening.

Publications:

2012:

1. Li W#, Shuai L#, Wan HF#, Dong MZ#, Wang M, Sang LS, Feng CJ, Luo GZ, Li TD, Li X, Wang LB, Zheng QY, Sheng C, Wu HJ, Liu ZH, Liu L, Wang L, Wang XJ, Zhao XY*, Zhou Q*. Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature 490(7420) (2012):407-411.（Equal contribution）

2. Liu ZC#, Wan HF#, Wang EY#, Zhao XY, Ding CH, Zhou SY, Li TQ, Shuai L, Feng CJ, Yu Y, Zhou Q*, Beaujean N*. Induced pluripotent stem induced cells show better constitutive heterochromatin remodeling and developmental potential after nuclear transfer than their parental cells. Stem Cell Development 21(16) (2012):3001-3009.

2013:

1. Shuai L#, Feng CJ#, Zhang HJ#, Gu Q, Jia YD, Wang L, Zhao XY, Liu ZH*, Zhou Q*. Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide (CBHA) treated androgenetic embryos. Chinese Science Bulletin 58(23) (2013):2862-2868.

2. Wan HF#, He ZQ#, Dong MZ, Gu TT, Luo GZ, Teng F, Xia BL, Li W, Feng CJ, Li X, Li TD, Shuai L, FuR, Wang L, WangXJ, Zhao XY* and Zhou Q*. Parthenogenetic haploid embryonic stem cells produce fertile mice. Cell research 23(2013):1330-1333.

2014:

1. Shuai L, Zhou Q*. Haploid embryonic stem cells serve as a new tool for mammalian genetic study. Stem Cell Research & Therapy 5(1) (2014):20.

2. Li W#, Li X#, Li TD#, Jiang MG#, Wan HF, Luo GZ, Feng CJ, Cui XL, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha JH, Xiao YM, Wang L, Liu ZH, Wang XJ, Zhao XY, Zhou Q*. Genetic modification and screening in the rat using haploid embryonic stem cells. Cell Stem Cell 14(3) (2014):404-414.

3. Shuai L., Li W, Wan H, Zhao XY, Wang L, Zhou Q. Generation of Mammalian Offspring by Haploid Embryonic Stem Cells Microinjection. Curr. Protoc. Stem Cell Biol. 31(2014):1A.6.1–1A.6.15.

2015:

1. Shuai L#, Wang Y#, Dong M#, Wang X#, Sang L, Wang M, Wan H, Luo G, Gu T, Yuan Y, Feng C, Teng F, Li W, Liu X, Li T, Wang L, Wang XJ, Zhao XY, Zhou Q*. 2015 Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. Journal of Molecular Cell Biology 7(4) (2015):326-337.

2. Li X#, Wang JQ#, Wang LY#, Wan HF, Li YF, Li TD, Wang YK, Shuai L, Mao YH, Cui XL, Wang L, Liu ZH, Li W, Zhou Q*. Co-participation of paternal and maternal genomes before the blastocyst stage is not required for full-term development of mouse embryos. Journal of Molecular Cell Biology 7(5) (2015):486-488.

2016:

1. Li X#, Cui XL#, Wang JQ#, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Wang XJ, Liu ZH, Li W*, Zhou Q*. Generation and Application of Mouse-Rat Allodiploid Embryonic Stem Cells. Cell 164(1-2) (2016):279-292.

2. Li.T.D.#, Shuai.L.#, Mao.J.J.#, Wang. X.P., Wang.M., Zhang X.X., Wang.L., Li.Y.N., Li.W., Zhou.Q*. Efficient production of fluorecent transgenic rats using the piggyback transposon. Scientific Reports. (2016): Doi:10.1038/srep33225.

3. Gu Q, Zhu H, Chen L, Shuai L, Fang J, Wu J, Liu L, Li W, Dai J, Hao J*, Zhou Q*l: Three dimensional collagen scaffolds promote iPSC induction with higher pluripotency. Protein Cell (2016), 7(11):844-848.

2017:

1. Li Y^#, Shuai L*. A versatile genetic tool: haploid cells. Stem Cell Res Ther (2017), 8(1): 197~197.

2018:

1. Gao Q^#, Zhang W^#, Ma L^#, Li X, Wang H, Li Y, Freimann R, Yu Y, Shuai L*, Wutz Anton*. Derivation of haploid neural stem cell lines by selection for a Pax6-GFP reporter. Stem Cells Dev (2018), 27(7):479-487.

2. Li Y^#, Li X^#, Wang H, Gao Q, Zhang J, Zhang W, Zhang Z, Li L, Yu Y*, Shuai L*. CRISPR/Cas9-edited Pax6-GFP reporter system facilitates the generation of mouse neural progenitor cells during differentiation. J Genet Genomics (2018), 45(5): 277-280.

3. Wang H^#, Zhang W, Yu J, Wu C, Gao Q, Li X, Li Y, Zhang J, Tian Y, Tan T, Ji W, Li L, Yu Y, Shuai L*. Genetic screening and multipotency in rhesus monkey haploid neural progenitor cells. Development (2018), 145(11). pii: dev160531.

4. Wang H^#, Zhang W^#, Gao Q^#, Cao X, Li Y, Li X, Min Z, Yu Y*, Guo Y*, Shuai L*. Extractive from Hypericum ascyron L promotes serotonergic neuronal differentiation in vitro. Stem Cell Res (2018), 31: 42-50.

2019:

1. Peng K^#, Li Xu^#, Wu C^#, Wang Y, Yu J, Zhang J, Gao Q, Zhang W, Zhang Q, Fan Yong, Yu Y, Shuai L*. Derivation of haploid trophoblast stem cells via conversion in vitro. iScience (2019), 11: 508-518.

2. Yao C, Zhang W, Shuai L*. The first cell fate decision in pre-implantation mouse embryos. Cell Regeneration (2019), 8: 51-57